4PXY
Crystal structure of a Putative thua-like protein (BACUNI_01602) from Bacteroides uniformis ATCC 8492 at 1.50 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 |
| Unit cell lengths | 132.666, 132.666, 75.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.356 - 1.500 |
| R-factor | 0.1374 |
| Rwork | 0.136 |
| R-free | 0.16090 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.681 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (July 4, 2012) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.356 | 1.550 | |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.037 | 0.019 | 0.371 |
| Number of reflections | 78045 | 14944 | 10525 |
| <I/σ(I)> | 13.18 | 36 | 1.9 |
| Completeness [%] | 90.5 | 94 | 70.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES pH 6, 10% polyethylene glycol 8000, 0.2M zinc acetate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
