4PXV
Crystal Structure of LysM domain from pteris ryukyuensis chitinase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-02-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.28213 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.686, 50.229, 92.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.150 - 1.800 |
| R-factor | 0.19322 |
| Rwork | 0.191 |
| R-free | 0.22908 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.072 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.108 | 0.380 |
| Number of reflections | 17318 | |
| <I/σ(I)> | 69.6 | 20 |
| Completeness [%] | 99.9 | 9.8 |
| Redundancy | 14 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05M zinc acetate dihydrate, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
