4PXT
Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 139.070, 139.070, 104.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.219 - 2.900 |
| R-factor | 0.2431 |
| Rwork | 0.242 |
| R-free | 0.27350 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.887 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.219 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.112 | 0.753 |
| Number of reflections | 12639 | |
| <I/σ(I)> | 11.9 | 3.5 |
| Completeness [%] | 92.4 | 100 |
| Redundancy | 11.1 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4-6 % (+/-) 2-Methyl-2,4 pentanediol (MPD), 100mM MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
