4PXK
Crystal structure of Haloarcula marismortui bacteriorhodopsin I D94N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 102.854, 102.854, 60.003 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.765 - 2.500 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fbz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.712 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.949 | |
| Number of reflections | 12828 | |
| Completeness [%] | 98.7 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 295 | 0.1M Tris, 2.6M ammonium sulfate, pH 8.8, in meso crystallization, temperature 295K |
