4PVK

Phl p 4 I153V N158H variant, a glucose oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-14
Detector	PSI PILATUS 6M
Wavelength(s)	0.915
Spacegroup name	P 61 2 2
Unit cell lengths	117.370, 117.370, 201.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.272 - 1.300
R-factor	0.142
Rwork	0.141
R-free	0.15950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.324
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (mr)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.400
High resolution limit [Å]	1.300	1.300
Number of reflections	197683
<I/σ(I)>		0.92
Completeness [%]	99.4	97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	7 mg/mL Protein in 20mM Tris + 70% Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K