4PVJ
Phl p 4 I153V variant, a glucose oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-04 |
| Detector | Pilatus 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 117.740, 117.740, 201.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.429 - 1.800 |
| R-factor | 0.1536 |
| Rwork | 0.152 |
| R-free | 0.18570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (mr) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 76859 | |
| <I/σ(I)> | 1.14 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 7 mg/mL Protein in 20mM Tris + 70% Tacsimate pH 7.0 , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
