4PVH
Phl p 4 N158H variant, a glucose dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 118.040, 118.040, 201.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.564 - 1.400 |
| R-factor | 0.1456 |
| Rwork | 0.144 |
| R-free | 0.16720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.349 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (mr) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 159575 | |
| <I/σ(I)> | 1.29 | |
| Completeness [%] | 98.7 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 7 mg/mL Protein in 20mM Tris + 70% Tacsimate , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
