4PVA
Crystal structure of GH62 hydrolase from thermophilic fungus Scytalidium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 31 |
| Unit cell lengths | 72.004, 72.004, 61.215 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.490 - 1.230 |
| R-factor | 0.1439 |
| Rwork | 0.143 |
| R-free | 0.16211 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.897 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.250 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.044 | |
| Number of reflections | 97754 | |
| <I/σ(I)> | 30 | |
| Completeness [%] | 94.7 | 52.2 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1 M Tris pH 8.5, 2.2 M ammonium di-hydrate phosphate, 12% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
