4PV9
Crystal Structure of H2Kb-Q600V complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.956 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.470, 90.750, 89.520 |
| Unit cell angles | 90.00, 111.58, 90.00 |
Refinement procedure
| Resolution | 61.810 - 2.000 |
| R-factor | 0.1755 |
| Rwork | 0.173 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zsv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.200 | |
| High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
| Rmerge | 0.097 | 0.042 | 0.441 |
| Number of reflections | 65401 | 477 | 16113 |
| <I/σ(I)> | 12.1 | 26.16 | 4.28 |
| Completeness [%] | 97.7 | 84.3 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 16-24% PEG 3350 and 0.2M Na acetate, 0.1M Na-cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
