4PV2
Crystal structure of potassium-dependent plant-type L-asparaginase from Phaseolus vulgaris in complex with K+ and Na+ cations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-09 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.827 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.885, 102.632, 123.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.770 - 1.790 |
| R-factor | 0.1643 |
| Rwork | 0.164 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pu6 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.373 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.770 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.053 | 0.630 |
| Number of reflections | 68445 | |
| <I/σ(I)> | 16.33 | 1.98 |
| Completeness [%] | 99.1 | 98.2 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 20% PEG3350, 0.1M bis tris propane, 0.2M sodium nitrate , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
