4PUS
Crystal Structure of Influenza A Virus Matrix Protein M1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-02-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.300, 37.346, 94.974 |
| Unit cell angles | 90.00, 102.44, 90.00 |
Refinement procedure
| Resolution | 27.990 - 2.200 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1EA3 AND 1AA7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.990 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.045 | 0.237 |
| Number of reflections | 19111 | |
| <I/σ(I)> | 15.2 | 4.7 |
| Completeness [%] | 99.1 | |
| Redundancy | 3.49 | 3.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 298 | protein in 55 mM phosphate, 0.2 M sodium chloride, 2 mM TCEP, pH 4.0, precipitant: 15% PEG3000, 75 mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
