4PUN
tRNA-Guanine Transglycosylase (TGT) Apo-Structure pH 7.8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97627 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.500, 64.650, 69.930 |
Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
Resolution | 45.006 - 1.250 |
R-factor | 0.14 |
Rwork | 0.139 |
R-free | 0.16150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.012 |
RMSD bond angle | 1.483 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.300 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 107127 | |
<I/σ(I)> | 18 | 2.7 |
Completeness [%] | 96.8 | 95.3 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 100 mM MES, 10% DMSO, 13% PEG 8000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |