4PU7

Shewanella oneidensis Toxin Antitoxin System Antitoxin Protein HipB Resolution 1.85

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	200
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	53.460, 72.950, 86.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.120 - 1.850
R-factor	0.1875
Rwork	0.186
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dnv
RMSD bond length	0.004
RMSD bond angle	0.680
Refinement software	PHENIX ((phenix.refine: dev_1287))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.120
High resolution limit [Å]	1.850
Number of reflections	27424
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	0.1M Na HEPES pH7.5, 10% v/v 2-propanol, 20% PEG 4000, VAPOR DIFFUSION, temperature 293K