4PU5
Shewanella oneidensis Toxin Antitoxin System Toxin Protein HipA Bound with AMPPNP and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2012-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.700, 75.830, 110.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.607 - 1.834 |
| R-factor | 0.1568 |
| Rwork | 0.156 |
| R-free | 0.17540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dnv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.948 |
| Refinement software | PHENIX ((phenix.refine: dev_1287)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.610 | |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 45100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.2 M sodium chloride, 0.1 M HEPES sodium, pH 7.0, 25% PEG4000, VAPOR DIFFUSION, temperature 293K |
