4PTU
Crystal Structure of anti-23F strep Fab C05 with rhamnose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2013-11-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.150, 48.740, 79.310 |
| Unit cell angles | 90.00, 120.20, 90.00 |
Refinement procedure
| Resolution | 41.454 - 1.511 |
| R-factor | 0.1803 |
| Rwork | 0.179 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hie |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.157 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 1.550 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 68342 | |
| <I/σ(I)> | 29.22 | 6.12 |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1M Na Acetate, 20% PEG 1500, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
