4PTD
PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT D274N
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-11-25 |
| Detector | XENTRONICS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.700, 44.600, 153.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.300 - 2.300 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD-TYPE PI-PLC |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.900 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.300 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.053 | 0.164 |
| Total number of observations | 21317 * | |
| Number of reflections | 12218 * | |
| <I/σ(I)> | 14.3 | 3.6 |
| Completeness [%] | 86.6 | 71.3 * |
| Redundancy | 1.57 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 18 (mM) | |
| 3 | 1 | drop | 0.0045 (%) | ||
| 4 | 1 | drop | PEG600 | 16.36 (%) | |
| 5 | 1 | drop | trisodium citrate | 0.09 (M) | |
| 6 | 1 | drop | 6.45 (mM) | ||
| 7 | 1 | reservoir | 1.5-2.25 (M) |
