4PT7
Structure of initiator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 199.900, 199.900, 131.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 109.900 - 2.350 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THE PSK41 REPA NTD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 109.900 |
| High resolution limit [Å] | 2.350 |
| Number of reflections | 55300 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 15% PEG 3000, 5% PEG 400, 14 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
