4PSV
Mycobacterium tuberculosis RecA phosphate bound room temperature structure I-RT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-09-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 108.416, 108.416, 72.748 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.110 - 2.600 |
| R-factor | 0.15825 |
| Rwork | 0.154 |
| R-free | 0.19588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g19 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.232 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.056 | 0.470 |
| Number of reflections | 14941 | |
| <I/σ(I)> | 25.9 | 3.4 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 30% PEG 3350, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
