4PSL

Crystal structure of pfuThermo-DBP-RP1 (crystal form I)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-21
Detector	RAYONIX MX-225
Wavelength(s)	0.97962, 0.98003, 0.97185
Spacegroup name	P 64
Unit cell lengths	136.636, 136.636, 122.055
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.160 - 3.500
R-factor	0.21015
Rwork	0.207
R-free	0.26693
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.158
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.850
High resolution limit [Å]	3.500	3.500
Completeness [%]	99.6	99.6
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	50 mM sodium cacodylate, pH 6.4, 2.3 M (NH4)2SO4 and 10 mM MgSO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K