4PSH
Structure of holo ArgBP from T. maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9799 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 79.482, 79.482, 434.232 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.17249 |
| Rwork | 0.169 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.835 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 29302 | |
| Completeness [%] | 97.5 | 75.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.2M Potassium acetate, 20%(w/v) Polyethylene glycol 3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
