4PQZ

Crystal structure of swt1 C-terminal domain from yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-10
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	61.518, 61.518, 84.967
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.322 - 2.300
R-factor	0.2118
Rwork	0.212
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SEMET DERIVATIVE OF L379M MUTANT
RMSD bond length	0.009
RMSD bond angle	1.141
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.056	0.395
Number of reflections	8407
<I/σ(I)>	61.89	5.99
Completeness [%]	98.9	100
Redundancy	10.6	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	0.1 M sodium acetate, 2 M sodium formate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K