4PQO

Structure of the human SNX14 PX domain in space group I41

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2013
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	I 41
Unit cell lengths	86.840, 86.840, 74.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.098 - 2.550
R-factor	0.1882
Rwork	0.183
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.260
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.405
High resolution limit [Å]	2.550	2.550
Number of reflections	9067
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	1.8 M ammonium citrate tribasic, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K