4PQI
Crystal structure of glutathione transferase lambda3 from Populus trichocarpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28255 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.524, 77.013, 82.636 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.507 - 1.950 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qag |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.108 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.510 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.047 | 0.590 |
| Number of reflections | 19546 | |
| <I/σ(I)> | 16.2 | 2.2 |
| Completeness [%] | 98.6 | 97.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 25% (w/v) PEG4000, 200 mM calcium chloride and 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
