4PQ1
Crystal structure and functional implications of a DsbF homologue from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.103, 36.162, 62.522 |
| Unit cell angles | 90.00, 67.89, 90.00 |
Refinement procedure
| Resolution | 36.181 - 2.097 |
| R-factor | 0.1868 |
| Rwork | 0.184 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.088 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.181 | 4.000 | 2.200 |
| High resolution limit [Å] | 2.097 | 3.180 | 2.090 |
| Number of reflections | 18081 | ||
| Completeness [%] | 93.2 | 99 | 86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 287.15 | 0.1M tris-HCl, 30% PEG 4K, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 287.15K |
