4PPG
Mycobacterium tuberculosis RecA citrate bound low temperature structure IIA-BR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 61 |
| Unit cell lengths | 106.829, 106.829, 70.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.18889 |
| Rwork | 0.183 |
| R-free | 0.24769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g19 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.978 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.920 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.199 | 0.880 |
| Number of reflections | 9281 | |
| <I/σ(I)> | 15.8 | 3 |
| Redundancy | 13.1 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 15% PEG 3350, 10% PEG 5000 MME, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE , pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
