4PPD
PduA K26A, crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 235.340, 235.340, 235.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.205 - 2.400 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.580 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX (dev_1555) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 83.205 | 83.205 | 2.460 |
| High resolution limit [Å] | 2.400 | 10.730 | 2.400 |
| Rmerge | 0.075 | 0.030 | 1.067 |
| Number of reflections | 42125 | 514 | 3092 |
| <I/σ(I)> | 32.82 | 88.74 | 4.24 |
| Completeness [%] | 100.0 | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 125 mM cesium sulfate, 1.8 M ammonium sulfate, pH 9.0, vapor diffusion, hanging drop, temperature 298K |
