4POU
VHH-metal in Complex with RNase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Collection date | 2010-07-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.425, 54.745, 43.958 |
| Unit cell angles | 90.00, 108.26, 90.00 |
Refinement procedure
| Resolution | 21.290 - 1.750 |
| R-factor | 0.18457 |
| Rwork | 0.182 |
| R-free | 0.22987 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.545 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.270 |
| Number of reflections | 51284 |
| Completeness [%] | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298.15 | 25% PEG 3,350, 0.1M Bis-Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
