4PON

The crystal structure of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-24
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97958
Spacegroup name	P 21 21 21
Unit cell lengths	48.859, 76.570, 100.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.20192
Rwork	0.200
R-free	0.23595
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eey
RMSD bond length	0.015
RMSD bond angle	1.460
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	30744
Completeness [%]	99.3	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30-36% PEG200/5% PEG2000/0.1 M sodium cacodylate (pH 6.5) or 30-36% PEG200/5% PEG2000/0.1 M Hepes (pH 7.0), VAPOR DIFFUSION, SITTING DROP, temperature 291K