4PO9
Mycobacterium tuberculosis RecA glycerol bound low temperature structure IIC-BR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-12 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 61 |
Unit cell lengths | 107.090, 107.090, 71.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.740 - 2.750 |
R-factor | 0.20287 |
Rwork | 0.197 |
R-free | 0.25466 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g19 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.011 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.140 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.172 | 0.882 |
Number of reflections | 12222 | |
<I/σ(I)> | 16.2 | 3 |
Redundancy | 12.3 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 15% PEG 3350, 10% PEG 5000 MME, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |