4PO5

Crystal structure of allophycocyanin B from Synechocystis PCC 6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-06
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	I 4 2 2
Unit cell lengths	184.293, 184.293, 260.954
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.579 - 1.751
R-factor	0.1714
Rwork	0.170
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b33
RMSD bond length	0.009
RMSD bond angle	1.507
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1610))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.750
Rmerge	0.052
Number of reflections	219625
<I/σ(I)>	23.8
Completeness [%]	98.6
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M ammonium sulfate, 0.1M sodium cacodylate (pH 6.5) and 30% (w/v) PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K