4PN8
A de novo designed pentameric coiled coil CC-Pent.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2014-02-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.500, 55.580, 61.400 |
| Unit cell angles | 90.00, 106.74, 90.00 |
Refinement procedure
| Resolution | 29.735 - 2.000 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.882 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.740 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.417 |
| Number of reflections | 24003 | |
| <I/σ(I)> | 6.9 | 2.1 |
| Completeness [%] | 95.4 | 96.6 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.1 M MES, 10 % w/v PEG 5000 MME, 12% w/v 1-propanol |
