4PN7

Crystal Structure of the TFIIH p34 N-terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91985
Spacegroup name	F 41 3 2
Unit cell lengths	257.110, 257.110, 257.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.522 - 2.801
R-factor	0.2197
Rwork	0.219
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.783
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	148.443	77.522	2.950
High resolution limit [Å]	2.801	8.850	2.800
Rmerge		0.064	2.598
Rmeas	0.155
Rpim	0.019	0.010	0.297
Total number of observations	1305233	34950	201698
Number of reflections	18512
<I/σ(I)>	20.9	38.1	2.9
Completeness [%]	100.0	99.9	100
Redundancy	70.5	49.6	76.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Crystals grew from protein solution in 20 mM Tris-HCl pH 8.0, 150 mM KCl and 1 mM TCEP over a reservoir containing the protein buffer including 50 - 500 mM NaCl