4PMY
Crystal structure of GH10 endo-b-1,4-xylanase (XynB) from Xanthomonas axonopodis pv citri complexed with xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.866, 48.201, 77.798 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 40.970 - 1.601 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.22680 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.016 |
| Data reduction software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 | |
| High resolution limit [Å] | 1.600 | 4.770 | 1.600 |
| Rmerge | 0.095 | 0.029 | 0.589 |
| Rmeas | 0.112 | 0.034 | 0.693 |
| Total number of observations | 246951 | ||
| Number of reflections | 69577 | 2747 | 10910 |
| <I/σ(I)> | 11.54 | 35.21 | 2.55 |
| Completeness [%] | 98.7 | 99.1 | 96.5 |
| Redundancy | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Polyethylene glycol 8,000 |
