4PMO
Crystal structure of the Mycobacterium tuberculosis Tat-secreted protein Rv2525c, monoclinic crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.442, 73.679, 74.415 |
| Unit cell angles | 90.00, 130.85, 90.00 |
Refinement procedure
| Resolution | 36.950 - 1.330 |
| R-factor | 0.15027 |
| Rwork | 0.149 |
| R-free | 0.16493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pmn |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.751 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.610 | 1.350 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.066 | 0.640 |
| Number of reflections | 90524 | |
| <I/σ(I)> | 11.9 | 1.8 |
| Completeness [%] | 97.3 | 75.4 |
| Redundancy | 3.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 2 M Na formate, 100 mM sodium Acetate pH 4.6 |
