4PMB
Crystal structure of Staphylcoccal nuclease variant Delta+PHS I92S at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-05-07 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.031, 59.964, 38.017 |
Unit cell angles | 90.00, 93.63, 90.00 |
Refinement procedure
Resolution | 37.940 - 1.800 |
R-factor | 0.1979 |
Rwork | 0.196 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.823 |
Data reduction software | SAINT (2008/2 for Windows) |
Data scaling software | SAINT |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.960 | 59.960 | 1.830 |
High resolution limit [Å] | 1.800 | 4.980 | 1.800 |
Rmerge | 0.035 | 0.049 | 0.227 |
Total number of observations | 120929 | ||
Number of reflections | 12978 | 650 | 464 |
<I/σ(I)> | 18.95 | 49.69 | 3.54 |
Completeness [%] | 99.9 | 100 | 100 |
Redundancy | 9.08 | 14.28 | 5.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% MPD, 25mM potassium phosphate, calcium chloride, pdTp |