4PMA
Crystal structure of CTX-M-14 S70G:S237A:R276A beta-lactamase at 1.39 Angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2011-02-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.742, 62.380, 86.701 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.985 - 1.399 |
R-factor | 0.1541 |
Rwork | 0.153 |
R-free | 0.18130 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.083 |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.390 |
Number of reflections | 46446 |
<I/σ(I)> | 27.5 |
Completeness [%] | 99.9 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | Ammonium sulfate, PEG 4000 |