4PM6

Crystal structure of CTX-M-14 S70G beta-lactamase at 1.56 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2014-04-21
Detector	RIGAKU
Wavelength(s)	1.5
Spacegroup name	P 21 21 21
Unit cell lengths	41.550, 62.280, 86.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.096 - 1.560
R-factor	0.1424
Rwork	0.141
R-free	0.16180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ylt
RMSD bond length	0.006
RMSD bond angle	1.086
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	22.100
High resolution limit [Å]	1.560
Number of reflections	32576
<I/σ(I)>	39.4
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	Ammonium Sulfate, PEG 4000