4PM4

Structure of a putative periplasmic iron siderophore binding protein (Rv0265c) from Mycobacterium tuberculosis H37Rv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	69.551, 65.434, 72.330
Unit cell angles	90.00, 116.41, 90.00

Refinement procedure

Resolution	29.204 - 2.200
R-factor	0.2055
Rwork	0.203
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mdy
RMSD bond length	0.003
RMSD bond angle	0.787
Refinement software	PHENIX ((phenix.refine: dev_1555))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	28285
<I/σ(I)>	10.4	2.3
Completeness [%]	94.5	98.4
Redundancy	2.4	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Reservoir solution: 3.0 M ammonium sulfate, 95 mM citric acid pH 5.0. Protein buffer: 20 mM Tris, pH 8.0, 300 mM NaCl, 10% glycerol