4PLM

Crystal Structure of Chicken Netrin-1 (LN-LE3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	298
Detector technology	CCD
Collection date	2000-01-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	62.627, 62.627, 269.788
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.639 - 2.801
R-factor	0.1989
Rwork	0.192
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.401
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.085	0.036
Total number of observations	92454
Number of reflections	17693
<I/σ(I)>	11.3
Completeness [%]	98.8	93.8	97.8
Redundancy	5.2	4.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	NaoAc, NaCacodylate