4PLK
Hepatitis E Virus E2s domain (Genotype I) in complex with a neutralizing antibody 8G12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.196, 191.993, 192.584 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 33.992 - 4.000 |
| R-factor | 0.2662 |
| Rwork | 0.264 |
| R-free | 0.29610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.580 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.992 |
| High resolution limit [Å] | 4.000 |
| Number of reflections | 31821 |
| <I/σ(I)> | 5.8 |
| Completeness [%] | 90.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 12% PEG 5000, 0.05M NaH2PO4 |
