4PL9
Structure of the catalytic domain of ETR1 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.998 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 76.260, 83.120, 91.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.560 - 1.900 |
R-factor | 0.1483 |
Rwork | 0.146 |
R-free | 0.18160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.319 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXDE |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 23386 | |
<I/σ(I)> | 26.3 | 5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.6 | 5.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 50 mM Cadmimum sulfate, 0.1 M HEPES pH 7.5, 1.0M Sodium acetate |