4PL6
Structure of the chromodomain of MRG2 in complex with H3K4me3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 |
| Unit cell lengths | 54.231, 54.231, 127.922 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.965 - 1.681 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.21380 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.680 |
| Number of reflections | 23375 |
| <I/σ(I)> | 36.1 |
| Completeness [%] | 96.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.1 M HEPES, PH 7.5, 9% PEG 6K, 5% MPD |
