4PL1

X-ray crystal structure of C118A RlmN from Escherichia coli with S-adenosylmethionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	55.627, 55.729, 254.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.850 - 2.580
R-factor	0.2651
Rwork	0.263
R-free	0.29980
RMSD bond length	0.006
RMSD bond angle	1.036
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.580
Number of reflections	24546
<I/σ(I)>	16.6
Completeness [%]	95.7
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 6000, HEPES, MPD