4PKX
The structure of a conserved Piezo channel domain reveals a novel beta sandwich fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.184, 37.254, 73.563 |
| Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
| Resolution | 33.233 - 2.540 |
| R-factor | 0.2198 |
| Rwork | 0.218 |
| R-free | 0.25910 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.966 |
| Data reduction software | XDS |
| Data scaling software | XDS (XDS) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.970 | 2.680 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.028 | 0.085 |
| Number of reflections | 7746 | |
| <I/σ(I)> | 28.4 | 11.2 |
| Completeness [%] | 93.6 | 75.4 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M phosphate-citrate buffer pH 4.8, 30% PEG3350, and 0.2 M Li2SO4 |
