4PK3
tubulin acetyltransferase complex with bisubstrate analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-01-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.163, 52.284, 50.142 |
| Unit cell angles | 90.00, 107.13, 90.00 |
Refinement procedure
| Resolution | 29.377 - 1.347 |
| R-factor | 0.1489 |
| Rwork | 0.147 |
| R-free | 0.17340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.731 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.380 |
| High resolution limit [Å] | 1.347 | 1.347 |
| Rmerge | 0.315 | |
| Number of reflections | 40567 | |
| <I/σ(I)> | 20.5 | 4.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1M Tris 8.5, 0.2M Ammonium Acetate, 25% Peg3350 |
