4PJM
Myosin VI motor domain in the Pi release state, space group P212121 - soaked with PO4 - located in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.490, 95.330, 103.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.740 - 2.050 |
| R-factor | 0.1622 |
| Rwork | 0.161 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.108 | 0.460 |
| Number of reflections | 57304 | |
| <I/σ(I)> | 17.63 | 6.38 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.92 | 14.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 277 | 4,5%PEG8k, 50mM MES pH 6,75, 1mM TCEP, 3% Isopropanol, 3% TertButanol, 50mM KSCN, 3% Glyc?rol then soaked with 150mM NaH2PO4 pH 7,5 and 25% Glyc?rol |
