4PJ8

Structure of human MR1-5-OP-RU in complex with human MAIT TRBV20 TCR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95
Spacegroup name	P 31 2 1
Unit cell lengths	113.570, 113.570, 227.981
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.070 - 3.300
R-factor	0.1658
Rwork	0.163
R-free	0.21280
RMSD bond length	0.010
RMSD bond angle	1.100
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.800
High resolution limit [Å]	3.300
Number of reflections	24580
<I/σ(I)>	7.9
Completeness [%]	94.8
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Bis-tris propane, sodium acetate