4PJ2
Crystal structure of Aeromonas hydrophila PliI in complex with Meretrix lusoria lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.933, 77.667, 69.380 |
| Unit cell angles | 90.00, 108.12, 90.00 |
Refinement procedure
| Resolution | 47.800 - 1.240 |
| R-factor | 0.1485 |
| Rwork | 0.148 |
| R-free | 0.16780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.289 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.800 |
| High resolution limit [Å] | 1.240 |
| Number of reflections | 144102 |
| <I/σ(I)> | 10.8 |
| Completeness [%] | 95.7 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M DL-malic acid/MES/Tris-base cocktail buffer at pH 5.0 and 25% w/v PEG 1,500 |
