4PIF
Crystal Structure of recombinant WT Banana Lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.07810 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.510, 94.492, 63.443 |
| Unit cell angles | 90.00, 97.47, 90.00 |
Refinement procedure
| Resolution | 24.890 - 1.700 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.062 | 0.030 | 0.309 |
| Total number of observations | 243152 | ||
| Number of reflections | 63903 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 98.7 | 99.3 | 96.9 |
| Redundancy | 3.8 | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | 10-20% PEG 8000, 0.05M Potassium Phosphate |
