4PI2
Crystal structure of particulate methane monooxygenase from Methylocystis sp. ATCC 49242 (Rockwell) soaked in zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.27816, 1.33765 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.857, 185.450, 192.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.330 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.28240 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.712 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.330 |
| Number of reflections | 51611 |
| <I/σ(I)> | 23.6 |
| Completeness [%] | 80.4 |
| Redundancy | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 10% PEG3000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M magnesium formate dihydrate |
